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Fine structure genetics : ウィキペディア英語版 | Fine structure genetics
Fine structure genetics encompasses a set of tools used to examine not just the mutations within an entire genome, but can be isolated to either specific pathways or regions of the genome. Ultimately, this more focused lens can lead to a more nuanced and interactive view of the function of a gene. ==Regional Mutagenesis==
Similar to forward genetics, regional mutagenesis seeks to saturate with insertions or point mutations, but instead of for the entire genome, it saturates only a small portion of the genome. By limiting the region in focus, researchers are then able to intensify the number of mutations within any genes or promoters within that regions, often illuminating more complicated functions than could be identified with a broader focus. Furthermore, such mutations can show how the specific structure of that region of a chromosome affect expression levels and function.〔A Primer of Genome Science, Third Edition. Greg Gibson and Spencer V. Muse. 2009. Sinauer Press〕 Such mutations are introduced in the same means as forward genetics, often through chemical induction or transposable element insertions. The creation of specific balancer chromosomes that are restrictive to only a small region of the genome can guarantee that mutations will only be isolated and reproduced only in that region.〔A Primer of Genome Science, Third Edition. Greg Gibson and Spencer V. Muse. 2009. Sinauer Press〕
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